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Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc mutant)

Name: Lipopolysaccharides (rough strains) from <I>Escherichia coli</I> J5 (Rc mutant)
Brand: SIGMA

Synonym(s):

LPS

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About This Item

CAS Number:

93572-42-0

UNSPSC Code:

12352201

EC Number:

297-473-0

NACRES:

NA.25

MDL number:

MFCD00164401

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Properties

biological source

Escherichia coli (J5 Rc mutant)

Quality Level

200

form

lyophilized powder

impurities

<3% Protein (Lowry)

color

white to faint beige

solubility

water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

Description

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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This Item	L2637	L2880	L4005
Sigma-Aldrich L5014 Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc mutant)	Sigma-Aldrich L2637 Lipopolysaccharides from Escherichia coli O55:B5	Sigma-Aldrich L2880 Lipopolysaccharides from Escherichia coli O55:B5	Sigma-Aldrich L4005 Lipopolysaccharides from Escherichia coli O55:B5
biological source Escherichia coli (J5 Rc mutant)	biological source Escherichia coli (O55:B5)	biological source Escherichia coli (O55:B5)	biological source Escherichia coli (O55:B5)
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
solubility water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow	solubility water: soluble	solubility water: soluble	solubility water: soluble
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
impurities <3% Protein (Lowry)	impurities <3% Protein	impurities <3% Protein (Lowry)	impurities 1-10% Protein (Lowry)

pictograms

GHS06

signalword

Danger

hcodes

H300

pcodes

P264 - P270 - P301 + P310 - P405 - P501

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000439787

0000476051

0000406509

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Activated T cells induce macrophages to produce NO and control Leishmania major in the absence of tumor necrosis factor receptor p55.

M Nashleanas et al.

Infection and immunity, 68(3), 1428-1434 (2000-02-26)

The ability to activate macrophages in vitro for nitric oxide production and killing of Leishmania major parasites is dependent on tumor necrosis factor, although L. major-infected mice lacking the TNF receptor p55 (TNFRp55(-/-) mice) or both the TNFRp55 and TNFRp75

Hypoxia-inducible factor-1α mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions.

Xiaoyu Zhang et al.

Oncology letters, 12(2), 1034-1040 (2016-07-23)

Hypoxia-inducible factor-1α (HIF-1α) and toll-like receptor 4 (TLR4) are involved in numerous mechanisms of cancer biology, including cell proliferation and survival; however the interaction of the two factors under hypoxic conditions remains unclear. The present study investigated the in vitro

TAK-242, a small-molecule inhibitor of Toll-like receptor 4 signalling, unveils similarities and differences in lipopolysaccharide- and lipid-induced inflammation and insulin resistance in muscle cells.

Sophie E Hussey et al.

Bioscience reports, 33(1), 37-47 (2012-10-12)

Emerging evidence suggests that TLR (Toll-like receptor) 4 and downstream pathways [MAPKs (mitogen-activated protein kinases) and NF-κB (nuclear factor κB)] play an important role in the pathogenesis of insulin resistance. LPS (lipopolysaccharide) and saturated NEFA (non-esterified fatty acids) activate TLR4

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Global Trade Item Number

SKU	GTIN
L5014-10MG	04061833274927
L5014-5MG	04061833957851
L5014-50MG	04061833274934

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