This product contains formamide, with the exact composition being proprietary. The Safety Data Sheet indicates that the formamide content is greater than or equal to 50% and less than 70%.
H7782
Hybridization solution
Synonym(s):
Hyb solution, Hybridization solution for in situ hybridization, Ready-to-use hybridization solution
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About This Item
UNSPSC Code:
12161503
EC Number:
200-842-0
NACRES:
NA.32
Pricing and availability is not currently available.
General description
In situ hybridization assays detect a specific nucleic acid sequence of a gene at the cellular level. Hybridization solution is used to enhance hybrid formation between the DNA probe and the target mRNA on tissue sections. It may be used for reconstituting and/or diluting the probe for in situ hybridization experiments, or as a negative control (without probe).
Ready-to-use hybridization solution optimized for in situ hybridization (ISH) procedures.
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This Item | |||
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| technique(s) FISH: suitable | technique(s) - | technique(s) Northern blotting: suitable, Southern blotting: suitable | technique(s) - |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| form liquid | form liquid (2x concentrate) | form - | form solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. room temp |
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signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
target_organs
Blood
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Does this hybridization mix contain 50% formamide?
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Is this buffer appropriate for Fluorescent in situ hybridization using probes labeled with ATTO by nick translation?
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This product has not been tested for the application. So, there is no reference data available.
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Is this buffer appropriate to use with all probe types? Will it work with labeled (DIG, DNP, Biotin, etc.) polynucleotide probes?
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The buffer is optimized for both DNA and RNA probes for in situ hybridization. It has been used with FITC and biotinylated probes.
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What is the composition of the Hybridization solution ( H7782)?
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The specific formulation of this product is considered proprietary but includes dextran sulfate and formamide in a sodium citrate solution.
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