C7930

Anti-CD3, T Cell antibody produced in rabbit

whole antiserum

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162554
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC (p), WB
• Citations: 45

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen 20 kDa (bovine and swine)
• contains: 15 mM sodium azide
• species reactivity: rat, canine, mouse, human, Psammomys (sand rat), sheep, feline, pig, chicken, bovine
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using enzyme pre-digested, formalin-fixed, paraffin-embedded human tonsil sections, western blot: 1:200 using mouse thymus extract
• UniProt accession no.: P07766
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CD3E(916) ; mouse ... Cd3e(12501) ; rat ... Cd3e(315609)

Description

General description

CD3 is a 20kDa glycoprotein expressed on surface of all human T lymphocytes. CD3 molecule is encoded by the gene mapped to human chromosome 11q23.3. The CD3 antigen is expressed during thymopoiesis. It is also present in the majority of T-CLL and T-lymphomas and in approximately 70% of T-ALL.

Immunogen

synthetic human CD3 peptide (residues 156-168 of the CD3-ε chain) coupled to porcine thyroglobulin. Coupling was performed by a modification of the reported method.

Application

Anti-CD3, T Cell antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence staining
• immunohistochemistry
• immunohistochemical analysis

Biochem/physiol Actions

CD3 is a human cell surface glycoprotein complex involved in signal transduction following binding of antigen receptor.

The antibody reacts with the proline-rich peptide sequence comprising 13 amino acids in the cytoplasmic domain of the CD3-ε chain. It detects normal, reactive and most neoplastic human T cells in routinely processed enzyme pre-digested, formalin-fixed, paraffin-embedded tissue sections. The antibody also reacts with human tissues fixed with Bouin′s solution and embedded in paraffin. Frozen sections and smears are also suitable for staining with this antibody. The antibody reacts with T cell areas in lymphoid tissues derived from various species.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg