The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf
C6295
Cell Freezing Medium-DMSO Serum free 1x
sterile-filtered, suitable for cell culture
Synonym(s):
cryopreservation medium
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Pricing and availability is not currently available.
sterility
sterile-filtered
Quality Segment
form
liquid
technique(s)
cell culture | mammalian: suitable, cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.
Application
Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.
Other Notes
Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
Xiangwen Zhan et al.
Biochemical and biophysical research communications, 501(3), 779-785 (2018-05-16)
The proto-oncogene PIM1 encodes Ser/Thr kinase and regulates cell growth, differentiation and apoptosis. However, more and more studies including ours have found that PIM1 can induce senescence in normal human diploid fibroblasts and behave as a tumor suppressor. But the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C6295-50ML | 04061833511114 |
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Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.
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