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Cholera Toxin B subunit

Name: Cholera Toxin B subunit
Brand: SIGMA

GM1-binding, lyophilized powder

Synonym(s):

CTB

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About This Item

UNSPSC Code:

12352200

PubChem Substance ID:

329774902

NACRES:

NA.77

MDL number:

MFCD02356522

Form:

lyophilized powder

Quality level:

200

Pricing and availability is not currently available.

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Properties

Product Name

Cholera Toxin B subunit, FITC conjugate, lyophilized powder

conjugate

FITC conjugate

Quality Level

200

form

lyophilized powder

mol wt

~12 kDa

composition

Protein, ~20% Lowry

extent of labeling

~1.0 mol FITC per mol protein

storage temp.

2-8°C

SMILES string

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

Description

Application

Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:

to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat[1]
to stain epididymal sperms of mice[2]
to stain peritoneal macrophages of mice[3]

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide

Analysis Note

Activity measured by ELISA using ganglioside GM₁ coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.

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1 of 1

This Item	C9972	C3741	C9903
Sigma-Aldrich C1655 Cholera Toxin B subunit	Sigma-Aldrich C9972 Cholera Toxin B subunit	Sigma-Aldrich C3741 Cholera Toxin B subunit	Sigma-Aldrich C9903 Cholera Toxin B subunit
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300
mol wt ~12 kDa	mol wt ~12 kDa	mol wt pentamer 57 kDa, ~12 kDa	mol wt ~12 kDa
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
composition Protein, ~20% Lowry	composition Protein, ~40% Lowry	composition -	composition Protein, ~5% Lowry
extent of labeling ~1.0 mol FITC per mol protein	extent of labeling -	extent of labeling -	extent of labeling -

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pictograms

GHS07

signalword

Warning

hcodes

H332,H412

pcodes

P261 - P271 - P273 - P304 + P340 + P312 - P501

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Journal of leukocyte biology (2021-02-03)

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Cellular uptake and intracellular localization of poly (acrylic acid) nanoparticles in a rainbow trout (Oncorhynchus mykiss) gill epithelial cell line, RTgill-W1.

Lindsey C Felix et al.

Aquatic toxicology (Amsterdam, Netherlands), 192, 58-68 (2017-09-18)

The ever-growing production of engineered nanoparticles (NPs) for use in many agricultural, commercial, consumer, and industrial applications will lead to their accidental or intentional release into the environment. Potential routes of environmental exposure include manufacturing or transport spills, disposal of

Cell penetrating peptide-modified poly(lactic-co-glycolic acid) nanoparticles with enhanced cell internalization.

Jill M Steinbach et al.

Acta biomaterialia, 30, 49-61 (2015-11-26)

The surface modification of nanoparticles (NPs) can enhance the intracellular delivery of drugs, proteins, and genetic agents. Here we studied the effect of different surface ligands, including cell penetrating peptides (CPPs), on the cell binding and internalization of poly(lactic-co-glycolic) (PLGA)

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Global Trade Item Number

SKU	GTIN
C1655-.5MG	04061833467916
C1655-250UG	04061832980911

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