AP0100

Alkaline Phosphatase Diethanolamine Activity Kit

sufficient for 100 tests

• Synonym(s): ALP Activity Kit
• NACRES: NA.26
• UNSPSC Code: 12352202

Properties

• usage: sufficient for 100 tests
• Quality Segment: 200
• storage temp.: 2-8°C

Description

General description

The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.

Application

Suitable for the measurement of alkaline phosphatase activity in a variety of samples

Alkaline Phosphatase Diethanolamine Activity Kit has been used:

• in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts
• for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells
• for determining ALP activity in the bone metastasis model of lung cancer
• for measuring ALP levels in colon tissue homogenates

Biochem/physiol Actions

Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.

Kit Components Also Available Separately

• Alkaline Phosphatase Diethanolamine Reaction Buffer

  SDS
• P4744

  Phosphatase substrate, powder

  SDS
• P6774

  Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous solution, ≥2,000 DEA units/mg protein

  SDS

Safety Information

• pictograms: GHS08,GHS05
• signalword: Danger
• hcodes: H315,H318,H334,H361fd,H373
• pcodes: P202 - P260 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• target_organs: Kidney,Liver,Blood
• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Hazard Classifications: Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 2 Oral