A7130
β-Amylase from barley
Type II-B, 20-80 units/mg protein (biuret)
Synonym(s):
1,4-α-D-Glucan maltohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-566-1
MDL number:
Specific activity:
20-80 units/mg protein (biuret)
Pricing and availability is not currently available.
type
Type II-B
Quality Level
form
powder
specific activity
20-80 units/mg protein (biuret)
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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sustainability
Greener Alternative Product
greener alternative category
, Enabling
shipped in
wet ice
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides [1]. β -Amylase, from barley, has been used to study how pressure and temperature affect catalytic activity [2].
Biochem/physiol Actions
β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline β-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity.[3]
Analysis Note
Crude
Other Notes
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| specific activity 20-80 units/mg protein (biuret) | specific activity ≥150 units/mg protein (biuret) | specific activity ~50 U/mg | specific activity 500-1,500 units/mg protein |
| form powder | form powder | form powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Related Content
QC Methods
Volker Heinz et al.
Biotechnology progress, 21(6), 1632-1638 (2005-12-03)
The depolymerization of starch by beta-amylase during exposure to hydrostatic pressure up to 700 MPa and within a temperature range from 20 to 70 degrees C has been investigated. Inactivation of the enzyme as well as alterations in conversion speed
Purification and characterisation of crystalline -amylase from barley.
K Visuri et al.
European journal of biochemistry, 28(4), 555-565 (1972-08-04)
Xin Li et al.
Journal of industrial microbiology & biotechnology, 38(11), 1837-1843 (2011-04-21)
A moderately halophilic strain LY9 with high amylolytic activity was isolated from soil sample obtained from Yuncheng, China. Biochemical and physiological characterization along with 16S rRNA sequence analysis placed the isolate in the genus Halobacillus. Amylase production started from the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A7130-250KU | 04061832841410 |
| A7130-10KU | 04061833383599 |
| A7130-50KU | 04061833383605 |