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Minimum Essential Medium

Name: Minimum Essential Medium
Brand: SIGMA

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Minimum Essential Medium Eagle, MEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

description

for research or for further manufacturing use

Quality Segment

500

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

components

L-glutamine: no
NaHCO₃: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO₂): yes

storage temp.

2-8°C

Description

General description

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO₂ gas.

Application

Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons.[1]

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This Item	51411C	51416C	51415C
SAFC 51412C Minimum Essential Medium	Sigma-Aldrich 51411C Minimum Essential Medium	SAFC 51416C Minimum Essential Medium	SAFC 51415C Minimum Essential Medium
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable, cell culture \| stem cell: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form liquid
components L-glutamine: no NaHCO₃: 2200 mg/L phenol red: 10.62 mg/L Earle’s salts (5% CO₂): yes	components Earle’s salts (5% CO₂): yes phenol red: 10.62 mg/L NaHCO₃: 2200 mg/L L-glutamine: 290 mg/L	components Earle’s salts (5% CO₂): yes L-glutamine: no	components Earle’s salts (5% CO₂): yes HEPES: 25 mM L-glutamine: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
description for research or for further manufacturing use	description for research or for further manufacturing use	description for research or for further manufacturing use	description for research or for further manufacturing use

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000486948

0000411539

0000381769

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Global Trade Item Number

SKU	GTIN
A7257-1G	04061833383933
A7257-500MG	04061833383940
A7257-5G	04061832088693
51412C-1000ML	04061832492780

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