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Urea

Name: Urea
Brand: SIAL

ACS reagent grade, ≥99%, solid, puriss. p.a., reag. Ph. Eur.

Synonym(s):

Carbamide, Carbonyldiamide

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About This Item

Linear Formula:

NH₂CONH₂

CAS Number:

57-13-6

Molecular Weight:

60.06

NACRES:

NA.21

PubChem Substance ID:

57648752

UNSPSC Code:

12352100

EC Number:

200-315-5

MDL number:

MFCD00008022

Beilstein/REAXYS Number:

635724

Assay:

≥99%, 99-101% (calc. on dry substance)

Form:

solid

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Properties

Product Name

Urea, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%

Quality Segment

200

agency

reag. Ph. Eur.

grade

ACS reagent, puriss. p.a.

assay

≥99%, 99-101% (calc. on dry substance)

form

solid

impurities

alkalic reac. substances, complies, ≤0.0005% heavy metals (as Pb), ≤0.05% ammonium (NH₄), ≤0.1% biuret

ign. residue

≤0.01% (as SO₄)

loss

≤1.0% loss on drying, 105°C, 1 h

mp

132-135 °C

solubility

H₂O: soluble 480 g/L at 20 °C

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl^-): ≤5 mg/kg, sulfate (SO₄^2-): ≤10 mg/kg

cation traces

Fe: ≤2 mg/kg

suitability

in accordance for appearance of solution, in accordance for identity

functional group

amine

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.

Urea is the most commonly used slow release nitrogen fertilizer.

Application

Urea has been used in the following studies:

Synthesis of SnO₂ nanoparticles.[1]
Synthesis of urethanes, ureides and semicarbazides.
Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain.[2]
Protein electrophoretic studies.[3]

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

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This Item	U5128	U1250	15604
Sigma-Aldrich 33247 Urea	Sigma-Aldrich U5128 Urea	Sigma-Aldrich U1250 Urea	Sigma-Aldrich 15604 Urea
assay ≥99%, 99-101% (calc. on dry substance)	assay ≤0.001% (Heavy Metals by ICP-OES, metals basis), 99.0-100.5%	assay ≥99.5%	assay 99.0-100.5%, 99.0-101.0% (calc. on dry substance)
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
solubility H₂O: soluble 480 g/L at 20 °C	solubility H₂O: soluble 480 g/L at 20 °C	solubility H₂O: 8 M	solubility H₂O: soluble 480 g/L at 20 °C
mp 132-135 °C	mp 132-135 °C (lit.)	mp 132-135 °C (lit.)	mp 132-135 °C
form solid	form solid	form pellets	form solid
density 1.335 g/mL at 25 °C (lit.)	density 1.335 g/mL at 25 °C (lit.)	density 1.335 g/mL at 25 °C (lit.)	density 1.335 g/mL at 25 °C (lit.)

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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