IF014
Interferon-β Protein, Recombinant human
Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a).
Synonym(s):
Fibroblast Interferon
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
eCl@ss:
32160405
Form:
liquid
Assay:
≥95% (one N-glycosylation site)
Biological source:
human
Recombinant:
expressed in CHO cells
Pricing and availability is not currently available.
biological source
human
Quality Level
recombinant
expressed in CHO cells
assay
≥95% (one N-glycosylation site)
form
liquid
specific activity
3.99 x 108 U/mg
manufacturer/tradename
Chemicon®
concentration
1 x 106 units/mL
technique(s)
cell culture | mammalian: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
-65 to -96°C
General description
Product Source: cDNA obtained from human fibroblast mRNA expressed in CHO cells.
Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a). Molecular weight is ~23 kDa.
Physical form
Frozen liquid in 50 mM NaOAc, pH 5.5, containing 0.1% BSA.
Preparation Note
After receipt, this product should be kept at -70°C or below for retention of full activity.
Thaw product vial by incubation cold tap water until just thawed. Thawed IFNβ should be
stored in working aliquots at -70°C for up to six months. Avoid multiple freeze/thaw cycles.
Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the
product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Thaw product vial by incubation cold tap water until just thawed. Thawed IFNβ should be
stored in working aliquots at -70°C for up to six months. Avoid multiple freeze/thaw cycles.
Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the
product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Analysis Note
Specific Activity: Determined by assaying inhibition of virus-induced cytopathic effect.In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%.The units are determined with respect to the international reference standard for human interferon beta (Hu-IFNbeta) provided by the National Institutes of Health.Unit of activity measured on VERO cells with vesicular stomatitis virus (VSV).
Other Notes
Replaces: IF008
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| biological source human | biological source human | biological source mouse | biological source mouse |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| recombinant expressed in CHO cells | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - |
| assay ≥95% (one N-glycosylation site) | assay 95% | assay ≥95% | assay >98% (SDS-PAGE and HPLC) |
| concentration 1 x 106 units/mL | concentration - | concentration 2560000 units/mL | concentration - |
| form liquid | form liquid | form buffered aqueous solution | form liquid |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| IF014 | 04053252470646 |