172426

Glucoamylase, Rhizopus sp.

Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. Useful for the enzymatic determination of starch.

• Sinónimos: Glucoamylase, Rhizopus sp., Glucan 1,4-α-glucosidase, Glucoamylase, Amyloglucosidase, γ-Amylase, Lysosomal α-glucosidase, exo-4,7-α-glucosidase
• Número de CAS: 9032-08-0
• Comisión internacional de enzimas: 3.2.1.3 (BRENDA, IUBMB)
• Número MDL: MFCD00081350
• Código UNSPSC: 12352202
• NACRES: NA.54

Propiedades

• origen biológico: fungus (Rhizopus spp.)
• Nivel de calidad: 100
• Formulario: lyophilized
• actividad específica: ≥10 unit/mg solid; ≥20 units/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• solubilidad: aqueous buffer: 1 mg/mL; water: 1 mg/mL
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Native glucoamylase from Rhizopus species. Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. It catalyzes the hydrolysis of 1,4-linked α-D-glucose residues in glucosylpolysaccharides, liberating glucose units. Useful for the enzymatic determination of starch.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will liberate 1 µmol glucose from maltopentaose per min at 37°C, pH 6.0.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Otras notas

Hiromi, K., et al. 1973. Biochim. Biophys. Acta302, 362.
Kelly, J.J. and Alpers, D.H. 1973. Biochim. Biophys. Acta315, 113.
Jeffrey, P.L., et al. 1970. Biochemistry9, 1403.
Brown, B. and Brown, D.H. 1965. Biochim. Biophys. Acta110, 124.
Tsujisaka, Y., et al. 1958. Nature181, 770.
Miller, K.D. and Copeland, W.H. 1956. Biochim. Biophys. Acta22, 193.
French, D. and Knapp, D.W. 1950. J. Biol. Chem.187, 463.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable