ROAMYGLL
Roche
Amyloglucosidase
from Aspergillus niger
Sinónimos:
Aspergillus niger, disaccharidase-type a-glucosidase
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About This Item
Productos recomendados
biological source
Aspergillus niger
Quality Level
form
lyophilized
specific activity
6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate)
~6 units/mg protein (At 25 °C with glycogen as the substrate.)
mol wt
97 kDa
packaging
pkg of 3,500 U (11202367001)
pkg of 500 U (11202332001)
manufacturer/tradename
Roche
optimum pH
4.6-4.8
storage temp.
2-8°C
General description
Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.
Specificity
Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.
Application
Amyloglucosidase from Aspergillus niger is used for the determination of starch.
Biochem/physiol Actions
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Working solution: Tris-buffered saline containing 0.1% Tween 20.
Storage conditions (working solution): 2 to 8 °C
The following concentrations should be taken as a guideline:
- Western blot: 1 to 10 μg/ml
Working solution: Tris-buffered saline containing 0.1% Tween 20.
Storage conditions (working solution): 2 to 8 °C
Other Notes
For life science research only. Not for use in diagnostic procedures.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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