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MilliporeSigma

R4001

Sigma-Aldrich

Ribonuclease A-agarose

ammonium sulfate suspension, 400-1,000 units/g agarose (One ml gel will yield 12-30 units)

Sinónimos:

Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

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About This Item

Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204

origen biológico

protein from bovine pancreas

Nivel de calidad

Materiales

beaded agarose

Formulario

ammonium sulfate suspension

actividad específica

400-1,000 units/g agarose (One ml gel will yield 12-30 units)

mol peso

~13,700

temp. de almacenamiento

2-8°C

Forma física

Suspension in 2.0 M (NH4)2SO4 pH 7.0

Nota de análisis

Electrophoretically and enzymatically assayed. RNase A and B contents determined by SDS-PAGE. Protein determined by E1%/280.

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A spectrophotometric method for the measurement of ribonuclease activity.
M KUNITZ
The Journal of biological chemistry, 164(2), 563-568 (1946-08-01)
Marka van Blitterswijk et al.
PloS one, 7(11), e48983-e48983 (2012-11-17)
Progressive muscular atrophy (PMA) and amyotrophic lateral sclerosis (ALS) are devastating motor neuron diseases (MNDs), which result in muscle weakness and/or spasticity. We compared mutation frequencies in genes known to be associated with MNDs between patients with apparently sporadic PMA
Chunju Li et al.
Journal of biotechnology, 162(2-3), 283-288 (2012-09-25)
Ribonuclease A (RNase A) is a therapeutic enzyme with cytotoxic action against tumor cells. Its clinical application is limited by the short half-life and insufficient stability. Conjugation of albumin can overcome the limitation, whereas dramatically decrease the enzymatic activity of
Yuen Lai Shek et al.
Biochemistry, 52(4), 672-680 (2013-01-09)
We report the first application of volume and compressibility measurements to characterization of interactions between cosolvents (osmolytes) and globular proteins. Specifically, we measure the partial molar volumes and adiabatic compressibilities of cytochrome c, ribonuclease A, lysozyme, and ovalbumin in aqueous
Yifei Kong et al.
Nanoscale, 5(3), 1009-1017 (2012-12-20)
Ultra-small gold nanoclusters (AuNCs) have unique size-dependent optical, electrical and chemical properties. They have emerged as a new nanomaterial with broad applications in optoelectronics, catalysis, biosensing, and bioimaging. Several strategies have been exploited to prepare AuNCs of different "magic number"

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