R4642
Ribonucleasa A from bovine pancreas
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
Sinónimos:
ARNasa, ARNasa A, Ribonucleasa pancreática, Ribonucleato 3′-pirimidinooligonucleotidohidrolasa
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About This Item
Número de CAS:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.53
Productos recomendados
biological source
bovine pancreas
Quality Level
grade
for molecular biology
form
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
mol wt
13.7 kDa
~13,700
concentration
20-40 mg/mL
suitability
suitable for
foreign activity
Endonuclease and exonuclease, none detected
NICKase and DNase, none detected
storage temp.
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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General description
La ARNasa, ribonucleasa A, es una endorribonucleasa que escinde los enlaces fosfodiéster del ARN monocatenario después de nucleótidos de pirimidina. Ataca el extremo 3′ fosfato (por ejemplo, el pG-pG-pC-pA-pG será escindido para dar pG-pG-pCp y A-pG). La mayor actividad la exhibe con ARN monocatenario. La ARNasa A es un polipéptido monocatenario que contiene cuatro puentes bisulfuro. Al contrario que la ARNasa B, no es una glucoproteína. Las ribonucleasas no hidrolizan el ADN porque el ADN carece de los grupos 2′-OH esenciales para la formación de los intermediarios cíclicos. La ARNasa A también puede hidrolizar el ARN de las muestras proteicas. La ARNasa A puede ser inhibida por alquilación de la His12 o la His119 y activada por sales de potasio y de sodio. La RNAasa se inhibe en presencia de iones de los metales pesados. La ARNasa también es inhibida competitivamente por el ADN.
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
Application
- La ribonucleasa A se utiliza para eliminar el ARN de las preparaciones de ADN plasmídico y ADN genómico, y de las muestras de proteínas.
- La ARNasa se utiliza también en el análisis de la secuencia del ARN y en los análisis de protección.
- La RNasa A se ha utilizado como herramienta para el diseño de medicamentos asistido por ordenador.
- La ARNasa A contribuye al análisis de las secuencias de ARN.
- La ARNasa A hidroliza el ARN contenido en las muestras proteicas.
- La ARNasa A apoya la purificación del ADN.
Suitable for:
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
- Plasmid DNA purification
Features and Benefits
Nuestra muy estable ribonucleasa A, ARNasa A, es idónea para la eliminación del ARN, la secuenciación del ARN y la purificación del ADN.
Components
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).
Unit Definition
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Analysis Note
Proteína determinada mediante E.
Other Notes
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
Application
Referencia del producto
Descripción
Precios
Related product
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Descripción
Precios
Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
Ribonucleasa A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
inhibitor
Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Protocolos
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Chromatograms
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