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P4600

Sigma-Aldrich

ReadyMix™ Taq PCR Reaction Mix

with MgCl2

Sinónimos:

PCR master mix, Taq PCR master mix

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About This Item

MDL number:
MFCD01635810
UNSPSC Code:
41106300
NACRES:
NA.55

form

liquid

usage

sufficient for 100 reactions

feature

dNTPs included
hotstart: no

concentration

1.5 units/reaction (50 μL reaction volume)

technique(s)

PCR: suitable

color

colorless

input

purified DNA

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

ReadyMix™ Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma′s high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma′s Taq DNA Polymerase.

Application

ReadyMix™ Taq PCR Reaction Mix has been used:
  • in the development and specificity test of the amplified fragment length polymorphism (AFLP) markers
  • in the PCR reaction for terminal restriction fragment length polymorphism (T-RFLP) analysis
  • in the molecular identification of yeasts
  • for routine PCR amplifications

Features and Benefits

  • Amplifies targets up to 7 kb in length

  • ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient formulation

  • ReadyMix format reduces setup time.

  • Reduced risk of contamination from multiple pipet steps.

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
Supplied with a vial of PCR grade water for dilution.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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Referencia del producto
Descripción
Precios

92210

Timestrip Plus™ 0 °C

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Assessment of fecal microflora changes in pigs supplemented with herbal residue and prebiotic
Samanta A K, et al.
PLoS ONE, 10(7), e0132961-e0132961 (2015)
Development of amplified fragment length polymorphism-derived functional strain-specific markers to assess the persistence of 10 bacterial strains in soil microcosms
Xiang S R, et al.
Applied and Environmental Microbiology, 76(21), 7126-7135 (2010)
Ashis Kumar Samanta et al.
PloS one, 10(7), e0132961-e0132961 (2015-07-16)
Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment. The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder)
"RNA walk" a novel approach to study RNA?RNA interactions between a small RNA and its target.
Lustig Y, Wachtel C, Safro M, Liu L and Michaeli S
Nucleic Acids Research, 38, 5-5 (2010)
Chitosan inactivates spoilage yeasts but enhances survival of Escherichia coli O157: H7 in apple juice
Kisko G, et al.
Journal of Applied Microbiology, 98(4), 872-880 (2005)
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Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Polymerase Chain Reaction (PCR) Basics

Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

Protocolos

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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