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V900412

Sigma-Aldrich

Tricine

Vetec™, reagent grade, ≥99%

Synonym(s):

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
5704-04-1
Molecular Weight:
179.17
Beilstein/REAXYS Number:
1937804
EC Number:
227-193-6
MDL number:
MFCD00004277
UNSPSC Code:
12161700
PubChem Substance ID:
329829821

grade

reagent grade

product line

Vetec™

assay

≥99%

form

crystalline powder

useful pH range

7.4-8.8

pKa (25 °C)

8.1

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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General description

Tricine is the most used electrophoresis buffer, which has a lower negative charge than glycine. This in turn helps it to migrate faster. Tricine is also used for the resuspension of cell pellets. Moreover, its high ionic strength leads to more movement of ions and less protein movement. This aids to separate low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that helps in the resolution of small proteins at lower acrylamide concentrations compared to glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.

Application

Buffer component for separation of low molecular weight peptides.

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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BIS-TRIS ≥98.0% (titration)

Sigma-Aldrich

B9754

BIS-TRIS

Encyclopedia of Dairy Sciences (2011)
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Christian Nilsson et al.
Electrophoresis, 31(3), 459-464 (2010-02-02)
Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in
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