T8158

Transferrin human

powder, BioReagent, suitable for cell culture

• Synonym(s): Siderophilin, partially saturated
• CAS Number: 11096-37-0
• EC Number: 234-318-8
• MDL number: MFCD00132442
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• product line: BioReagent
• assay: ≥98%
• form: powder
• concentration: ~25 mM
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested; hepatitis and HIV antibody, none detected
• solubility: H₂O: 50 mg/mL
• UniProt accession no.: P02787
• shipped in: ambient
• storage temp.: −20°C
• Gene Information: human ... TF(7018)

Description

General description

Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.

Application

Transferrin human has been used:

• to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity
• as a supplement in serum-containing/devoid medium for human thyroid cell culture
• as a target glycoprotein to develop an in-gel hydrazinolysis method

Biochem/physiol Actions

Transferrin mediates iron transportation from plasma to cells. The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin. The transferrin binds to specific cell surface receptors and are internalized by endocytosis. Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form.

Other Notes

Partially iron-saturated

Preparation Note

Heat treated at 60 °C for ten hours.

Analysis Note

Purity by agarose electrophoresis.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable