T5941

Trizma^® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

• Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein/REAXYS Number: 3675235
• EC Number: 214-684-5
• MDL number: MFCD00012590
• UNSPSC Code: 12161700
• PubChem Substance ID: 24900332
• NACRES: NA.25

Properties

• Quality Level: 400
• grade: BioPerformance Certified
• assay: ≥99.0% (titration)
• form: crystalline powder
• technique(s): DNA purification: suitable; cell culture | mammalian: suitable; electrophoresis: suitable; protein extraction: suitable
• impurities: DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected; endotoxin and total aerobic microbial count, tested; ≤0.5% water (Karl Fischer)
• color: white
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• mp: 150-152 °C
• solubility: H₂O: 667 mg/mL
• density: 1.39 g/cm³ at 20—25 °C; 1.40 g/cm³ at 20—25 °C
• absorption: ≤0.05 at 290 at 40%
• suitability: suitable for electrophoresis; suitable for immunohistochemistry; suitable for molecular biology
• application(s): diagnostic assay manufacturing; general analytical; life science and biopharma; sample preparation
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

General description

Tris HCl, also known as Trizma^® hydrochloride, is a widely used acidic buffer for preparing buffers within the physiological pH range of 7.3 to 7.5. With a pKa of 8.08 at 25°C, Tris HCl offers a pH range of 7.0 to 9.0, matching the typical pH of living organisms. As a result, Tris HCl is an essential component of buffer solutions used in various biological, cell culture, biochemistry, and molecular biology applications. It is important to note that neither Tris base nor Tris hydrochloride on their own can provide sufficient buffering capacity. Therefore, they are typically combined to create a buffer within the pH range of 7 to 9, ensuring effective pH control.

Tris buffers are indispensable in techniques like protein electrophoresis and western blotting. In many western blot protocols, a low ionic strength Tris buffer is employed for protein transfer. The transfer duration depends on the blotting apparatus and the size range of peptides under investigation. Tris-based buffers are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers.

Furthermore, Tris salts find extensive use in DNA agarose electrophoresis, particularly in the preparation of buffers like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA). These buffers play a pivotal role in various applications, including biochemistry, protein purification, and electrophoresis. Tris HCl is a versatile and essential for maintaining the pH of biological systems and ensuring the optimal performance of biochemical, cell culture, and molecular biology techniques.

Application

Tris-HCl hasbenn used

• as a washing and rinsing buffer in an immunohistochemical analysis(1)
• as a component of reducing sample buffer, lysis buffer and elution buffer, in a biochemical research involving gel electrophoresis and BioLayer interferometry measurement(2)

Features and Benefits

• Suitable for Molecular Biology and Cell Culture
• Can be used as a Buffer component, for Electrophoresis and Protein separation
• Tested for Endotoxins and Total Aerobic Microbial Count
• Free from DNase, NICKase, RNase, and Protease
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
• Highly soluble in water

Packaging

T5941-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves