648317

Tris, Hydrochloride, Molecular Biology Grade

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

• Synonym(s): Trizma^® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein/REAXYS Number: 3675235
• MDL number: MFCD00012590
• UNSPSC Code: 12161705
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 100
• assay: ≥99% (titration)
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• solubility: water: soluble
• cation traces: heavy metals (as Pb): ≤0.0002%
• absorption: ≤0.05 at 260 nm; ≤0.05 at 280 nm
• foreign activity: DNases, proteases, RNases, none detected
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

General description

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used biological buffer (absorbance: ≤0.05 at 260 nm and 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Warning

Toxicity: Standard Handling (A)

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable