SRP6427

FGFR1/CD331 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): BFGFR, CD331, CEK, FGFBR, FGFR-1, FLT2, H2, HBGFR, KAL2, N-SAM
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 40.1 kDa; observed mol wt 60-65 kDa by SDS-PAGE (DTT-reduced. Arg 22 is the predicted N-terminal.)
• packaging: pkg of 20 and 100 μg
• UniProt accession no.: P11362
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... FGFR1(2260)

Description

General description

Fibroblast growth factor receptor 1 (FGFR1) is also known as basic fibroblast growth factor receptor 1(BFGFR1), FMS-like tyrosine kinase, CD331, and is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. This protein is one of several fibroblast growth factor receptors, which are related proteins that are involved in important processes such as cell division, regulation of cell growth and maturation, formation of blood vessels, wound healing, and embryonic development. The FGFR1 protein spans the cell membrane, so that one end of the protein remains inside the cell and the other end projects from the outer surface of the cell. This positioning allows the FGFR1 protein to interact with specific growth factors outside the cell and to receive signals that help the cell respond to its environment. When growth factors attach to the FGFR1 protein, the receptor triggers a cascade of chemical reactions inside the cell that instruct the cell to undergo certain changes, such as maturing to take on specialized functions. The FGFR1 protein is thought to play an important role in the development of the nervous system. This protein may also help regulate the growth of long bones, such as the large bones in the arms and legs.

The FGFR1 (fibroblast growth factor receptor 1) gene is mapped to human chromosome 8p11.23. It codes for a cell-surface receptor tyrosine kinases and belongs to the FGFR family.

Biochem/physiol Actions

Fgfr1 (fibroblast growth factor receptor 1) protein prevents endothelial to mesenchymal transition by downregulating transforming growth factor β. Fgfr1 thus helps in maintaining normal vascularity, which is generally affected by diabetes condition. Upregulation of the FGFR1 gene indicates tumor progression and is observed in squamous cell carcinoma.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable