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SAB4301135

Sigma-Aldrich

Anti-FLAG Tag antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

1.4 mg/mL

technique(s)

western blot: 1:1000-1:5000 (Cell Lysate)

isotype

IgG

immunogen sequence

DYKDDDDK conjugated to KLH (synthetic peptide)

shipped in

wet ice

storage temp.

−20°C

General description

The FLAG epitope is composed of eight amino acids with the following sequence AspTyrLysAspAspAspAspLys. It helps in immunoaffinity purification of fusion proteins.

Specificity

The antibody detects transfected proteins contanining FLAG tag.

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH

Application

Anti-FLAG Tag antibody produced in rabbit has been used in:
  • western blotting
  • co-immunoprecipitation
  • immunofluorescence studies
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Learn more product details in our FLAG® application portal.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xuan Yang et al.
Cancer science, 110(7), 2133-2144 (2019-05-09)
Lymphoid-specific helicase (LSH) is overexpressed in tumor tissues and its overexpression is associated with poor prognosis in several cancers. However, the role and molecular mechanism of LSH in hepatocellular carcinoma (HCC) remains largely unknown. Herein, we report that LSH was
Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling
Cao Y, et al.
Biochimica et Biophysica Acta, 1863(12), 3148-3159 (2016)
Fajiu Wang et al.
American journal of translational research, 11(11), 7166-7185 (2019-12-10)
Mechanism by which CCNB1 regulates the cell cycle progression and its prognostic function in non-squamous non-small cell lung cancer (NSCLC) are necessary to be further elucidated. Data retrieved from gene expression omnibus (GEO) and cancer genome atlas (TCGA) combined with
Zhen Wei et al.
Nature communications, 9(1), 4468-4468 (2018-10-28)
The conversion of serine and glycine that is accomplished by serine hydroxymethyltransferase 2 (SHMT2) in mitochondria is significantly upregulated in various cancers to support cancer cell proliferation. In this study, we observed that SHMT2 is acetylated at K95 in colorectal
Huimin Zhao et al.
Plant physiology, 183(3), 1026-1034 (2020-04-25)
Chromatin immunoprecipitation (ChIP) is the gold-standard method for detection of interactions between proteins and chromatin and is a powerful tool for identification of epigenetic modifications. Although ChIP protocols for plant species have been developed, many specific features of plants, especially

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