Two of the FLAG-tagged proteins offered by Sigma-Aldrich are the Amino-terminal FLAG-BAP fusion protein (Product No. P7582) and the Carboxy-terminal FLAG-BAP fusion protein (Product No. P7457). Both of these proteins should work with the F2555 rabbit ANTI-FLAG.
F2555
Monoclonal ANTI-FLAG® antibody produced in rabbit
clone SIG1-25, ascites fluid
Synonym(s):
Anti-ddddk, Anti-dykddddk
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$543.00
In StockDetails
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About This Item
$543.00
In StockDetails
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SIG1-25, monoclonal
technique(s)
immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100
indirect ELISA: suitable
western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein
isotype
IgG
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
Specificity
Immunogen
Application
Learn more product details in our FLAG® application portal.
Physical form
Legal Information
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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What positive controls can be used for immunoblotting with Product F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit - clone SIG1-25?
1 answer-
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Why should I use Product No. F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit?
1 answer-
The main application for Product F2555 rabbit monoclonal is for immunoblotting after immunoprecipitation with M2, which is a mouse antibody. This enables using a secondary antibody (anti-rabbit) that will not bind the M2 mouse heavy (50 kD) and light chains (25 kD).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product F2555 a monoclonal or polyclonal FLAG® antibody?
1 answer-
F2555 is a monoclonal antibody, raised in rabbits. [Note: Nearly all of the monoclonal antibodies sold by Sigma-Aldrich are raised in mice.]
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Will Product No. F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit - clone SIG1-25, recognize an internal FLAG tag protein?
1 answer-
F2555 will recognize N-terminal and C-terminal FLAG fusion proteins.
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