SAB4200654

Monoclonal Anti-CHD1 antibody produced in rat

clone 2F11H5, purified from hybridoma cell culture

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 200
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2F11H5, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): flow cytometry: 5-10 μg/test using using HeLa cells; immunofluorescence: 20 μg/mL using using human HeLa cells.
• isotype: IgG2a
• UniProt accession no.: O14646
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CHD1(1105)

Description

General description

Monoclonal Anti-CHD1 (rat IgG2a isotype) is derived from the hybridoma 2F11H5 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide. Chromodomain-helicase-DNA-binding protein 1 (CHD1) is an ATP-dependent chromatin-remodeling factor expressed majorly in cerebellum and basal ganglia. CHD1 gene is mapped to human chromosome 5q15-q21.1. CHD1 comprises two chromodomains.

Specificity

Monoclonal Anti-CHD1 recognizes human CHD1.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human CHD1.

Application

Monoclonal Anti-CHD1 antibody produced in rat may be used in:

• immunofluorescence
• flow cytometry
• immunoblotting
• immunohistochemistry

Biochem/physiol Actions

Chromodomain-helicase-DNA-binding protein 1 (CHD1) interacts with the trimethylated lysine residues of histones and alters access of the transcriptional apparatus to its chromosomal DNA template. CHD1 is required for maintenance of open chromatin and pluripotency in embryonic stem cells. Missense mutation in the CHD1 gene is implicated in the Pilarowski-Bjornsson syndrome as well as in speech delay and facial dismorphism.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage,freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable