SAB4200337

Anti-phospho-RasGRP1(pThr¹⁸⁴) antibody, Mouse monoclonal

clone p-RasGRP1-1, purified from hybridoma cell culture

• Synonym(s): Anti-CALDAG-GEFI, Anti-CALDAG-GEFII, Anti-RAS guanyl releasing protein 1 (calcium and DAG-regulated) hRasGRP1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: p-RasGRP1-1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~90 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using whole extracts of HEK-293T cells overexpressing human RasGRP1
• isotype: IgG2a
• UniProt accession no.: O95267
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pThr184)
• Gene Information: human ... RASGRP1(10125) ; mouse ... Rasgrp1(19419) ; rat ... Rasgrp1(29434)

Description

General description

Monoclonal Anti-phospho-RasGRP1(pThr184) (mouse IgG2a isotype) is derived from the hybridoma p-RasGRP1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phospho-peptide corresponding to residues surrounding Thr184 of human RasGRP1 conjugated to KLH. RAS guanyl releasing protein 1 (RasGRP1) is a member of RasGRP family, characterized by the presence of a N-terminal Ras superfamily guanine nucleotide exchange factor (GEF) domain and and C-terminal calcium- and diacylglycerol (DAG)/phorbol ester-binding domains. It is an intracellular signaling protein. RasGRP1 has a N terminal guanine nucleotide exchange factor domain for Ras-like guanosine triphosphatases (GTPases) (RasGEFN/RasGEF), two EF hand motifs and a carboxy-terminal C1 domain.

Immunogen

a synthetic phospho-peptide corresponding to residues surrounding Thr¹⁸⁴ of human RasGRP1, conjugated to KLH. The corresponding sequence is identical in rat, mouse, monkey, bovine and canine RasGRP1.

Application

Monoclonal Anti-phospho-RasGRP1(pThr184) antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

RAS guanyl releasing protein 1 (RasGRP1) functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound guanosine diphosphate (GDP) for guanosine triphosphate (GTP). It activates the extracellular signal-regulated kinase (Erk)/mitogen-activated protein (MAP) kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable