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R406-05A

Sigma-Aldrich

Rat Osteoblasts: Rob, adult

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

rat bone (Rat bone)

Quality Level

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

morphology

osteoblast

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

arthritis; osteoporosis

shipped in

dry ice

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.


ROb from Cell Applications, Inc. have been used to evaluate a novel hydroxyapatite (HAp)/poly(l-lactic acid) (PLLA) nanocomposite nonwoven fabric, coated with calcined HAp nanocrystals as a material for bone regeneration and demonstrated better spreading on the nanocrystal-coated vs. uncoated surface (Yanagida, 2011). Characterization: Positive for bone mineralization.

Cell Line Origin

Bone

Application

Model for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatments.

Components

Rat Osteoblast Basal Medium that contains 10% FBS and 10% DMSO

Quality

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Preparation Note

  • 1st passage, >500,000 cells in Rat Osteoblast Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 10 population doublings

Subculture Routine

Please refer to the ROb Culture Protocol.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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