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biological source
rat brain (Rat brain)
Quality Level
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
morphology
glial
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
seizures
shipped in
dry ice
storage temp.
−196°C
General description
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Rat astrocytes (RA) are derived from rat cerebral cortex. Astrocytes are the most abundant cells in the central nervous system where they perform many functions, such as providing mechanical support and nutrients to neurons and removal of wastes from neurons; providing signaling to endothelial cells; regulating neurogenesis and controlling synaptic function. As the recognition of the importance of astrocytes in nervous system is increasing, RA serve as a useful in vitro model for exploring the diversity of astrocytes functions.Characterization: Stained positive for GFAP.Rat Astrocytes (RA) and Rat Brain Microvascular Endothelial Cells (RBMEC), both from Cell Applications, Inc., have been used to assess neuroprotective capabilities of bioenergy stabilizers in an in vitro model of stroke (Liu, 2013) and to investigates the effects of astrocytes on brain microvascular endothelial cells in relation to iron transport pathway (McCarthy, 2014).
Rat astrocytes (RA) are derived from rat cerebral cortex. Astrocytes are the most abundant cells in the central nervous system where they perform many functions, such as providing mechanical support and nutrients to neurons and removal of wastes from neurons; providing signaling to endothelial cells; regulating neurogenesis and controlling synaptic function. As the recognition of the importance of astrocytes in nervous system is increasing, RA serve as a useful in vitro model for exploring the diversity of astrocytes functions.Characterization: Stained positive for GFAP.Rat Astrocytes (RA) and Rat Brain Microvascular Endothelial Cells (RBMEC), both from Cell Applications, Inc., have been used to assess neuroprotective capabilities of bioenergy stabilizers in an in vitro model of stroke (Liu, 2013) and to investigates the effects of astrocytes on brain microvascular endothelial cells in relation to iron transport pathway (McCarthy, 2014).
Cell Line Origin
Brain
Application
Neuroscience research, mechanical & biochemical support, ion balance, nutrient provision, waste removal, cell signaling, regulation of neurogenesis, control of synaptic function, astrocyte function, gene expressino, injury repair, gliotransmitter release, neuronal excitability.
Components
Basal Medium containing 10% FBS & 10% DMSO
Quality
Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Preparation Note
- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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