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MAK208

Sigma-Aldrich

GPDH Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

Glycerol 3-Phosphate Dehydrogenase Activity Assay Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.84

detection method

colorimetric

storage temp.

−20°C

Gene Information

human ... GPD1(2819) , GPD2(2820)
mouse ... GPD1(14555) , GPD2(14571)
rat ... GPD1(60666) , GPD2(25062)

General description

Glycerol 3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) catalyzes the reversible conversion of dihydroxyacetone phosphate and NADH to glycerol-3-phosphate and NAD+. It forms a glycerol phosphate shuttle and facilitates the transfer of reduced equivalents across the mitochondria to the cytosol. GPDH is important for both carbohydrate and lipid metabolism. Mutations in GPDH gene result in transient infantile hypertriglyceridemia.

Application

GPDH (glyceraldhyde-3-phoshate dehydrogenase) activity assay kit has been used to measure the serum and hepatic concentrations of GPDH in rabbit.

Suitability

Suitable for the detection of Glycerol-3-Phosphate Dehydrogenase Activity in tissue and cell culture samples.

Principle

The Glycerol 3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit provides a simple and sensitive procedure for measuring GPDH activity in a variety of tissues and cells. GPDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of GPDH is the amount of enzyme required to generate 1.0 μmole of NADH per minute at pH 8 at 37 °C.

Kit Components Only

Product No.
Description
  • GPDH Assay Buffer
  • GPDH Substrate
  • GPDH Probe
  • NADH Standard
  • GPDH Positive Control

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

hcodes

H314,H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

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Certificates of Analysis (COA)

Lot/Batch Number
4A23K06400
5A02K06400
B1A160640V

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B. Elmahdi, MA Al-Omair, AA El-Bessoumy and SM El-Bahr.
Thomson I S I
International Journal of Pharmaceutics, 12(4), 429-434 (2016)
A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.
Joshi M, et al.
European Journal of Human Genetics, 22(10), 1229-1229 (2014)
Regulation of adenine nucleotide translocase and glycerol 3-phosphate dehydrogenase expression by thyroid hormones in different rat tissues.
DUMMLER K, et al.
The Biochemical Journal, 317(3), 913-918 (1996)
Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 ? resolution.
Alarcon D A, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 68(11), 1279-1283 (2012)

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