ZRB2383

Anti-GFAP Antibody, clone 2E8, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): GFAP
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit (recombinant)
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2E8, monoclonal; recombinant monoclonal
• description: 2E8 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 49.88 kDa; observed mol wt ~51 kDa
• species reactivity: human, rat, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable using 1:100; immunofluorescence: suitable using 1:100; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100; western blot: suitable using 1:1,000
• isotype: IgG
• UniProt accession no.: P14136
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... GFAP(2670)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 2E8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glial fibrilary acidic protein (GFAP). It targets an epitope with in 11 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 11 amino acids from the C-terminal region of human Glial fibrilary acidic protein (GFAP).

Application

Anti-GFAP, clone 2E8, ZooMAb, Cat. No. ZRB2383, is a recombinant Rabbit Monoclonal Antibody that specifically targets Glial fibrillary acidic protein (GFAP) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunfluorescence, and Western Blotting.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GFAP in human brain and rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GFAP in C6 glioblastoma cells and primary cultures of rat glial precursor cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GFAP in rat brain and human brain tissue sections.

Target description

Glial fibrillary acidic protein (UniProt: P14136; also known as GFAP) is encoded by the GFAP gene (Gene ID: 2670) in human. GFAP is a class-III intermediate filament that serves as a cell-specific marker that can distinguish astrocytes from other glial cells. GFAP is not detected outside the central nervous system. It is expressed in cells that lack fibronectin. GFAP is a good marker of astroglial injury where its levels are consistently upregulated. GFAP levels are also shown to be upregulated with age in rodents and humans. Hence, it can serve as a surrogate marker of aging in the brain. GFAP has a relatively slow metabolic turnover rate, which is consistent with its structural role. GFAP is composed of three distinct regions: an N-terminal head region, a central ROD region, and a C-terminal tail region. The ROD regions are highly conserved among intermediate filament proteins. However, the size and amino acid sequences of the head and tail regions can vary widely. Three different isoforms of GFAP have been described that are produced by alternative splicing. Mutations in GFAP gene are known to cause Alexander disease that is characterized by progressive failure of central myelination, usually leading to death within the first decade. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable