Lysing enzymes are also known as lytic enzymes or cell wall lytic enzymes.
Application
Lysing Enzymes from Aspergillus sp. has been used to digest the cell walls to create pulse-field gel plugs.
Lysing Enzymes from Aspergillus sp. has been used:
to digest the cell wall of Schizosaccharomyces pombe[1]
to lyse the mycelium cell wall of Trichoderma harzianum[2]
Biochem/physiol Actions
Lysing enzymes are a group of enzymes that can break down the cell walls of microorganisms. These enzymes play a crucial role in various biological processes, including cell lysis, tissue dissociation, and protein extraction. Lysing enzymes are commonly used in research, biotechnology, and clinical applications. They are especially useful in DNA extraction, where they help to release genetic material from cells by breaking down the cell walls. This enables efficient isolation and purification of DNA for downstream analyses. Cellulases are a class of enzymes that degrade cellulose, a major component of plant cell walls, into simpler sugars. These sugars can be utilized as raw materials to produce biofuels, biobased chemicals, plastics, and various other materials. Pectinases are a group of enzymes that hydrolyze pectic substances found in microorganisms and higher plants. They can break down the pectin-containing compounds synthesized by plants and microbes.
Translesion synthesis polymerases (TLSPs) are non-essential error-prone enzymes that ensure cell survival by facilitating DNA replication in the presence of DNA damage. In addition to their role in bypassing lesions, TLSPs have been implicated in meiotic double-strand break repair in
Pectinase from Microorganisms and Its Industrial Applications
Haile S and Ayele A
TheScientificWorldJournal (2022)
Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics in Schizosaccharomycespombe
Mastro TL, et al.
Journal of Cell Science (2020)
Unleashing the Promise of Biotechnology to Help Heal, Fuel, and Feed the World
Greenwood JC
Journal of biological and chemical chronicles (2014)
New strains obtained after UV treatment and protoplast fusion of native Trichoderma harzianum: their biocontrol activity on Pyrenochaeta lycopersici
Besoain X A, et al.
Electronic journal of Biotechnology, 10(4), 604-617 (2007)
What is the working condition and concentration of this enzyme, especially for filamentous fungi cell wall digestion
1 answer
Technical Support
·2 years ago
The working conditions and concentration of this enzyme is not tested. This process will have to be validated by the end-user It may be helpful to view the peer-reviewed articles:
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