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I9271

Sigma-Aldrich

5-(Iodoacetamido)fluorescein

≥90% purity (HPLC), powder

Synonym(s):

5-IAF

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100 MG
$537.00

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25 MG
$162.00
100 MG
$537.00

About This Item

Empirical Formula (Hill Notation):
C22H14INO6
CAS Number:
63368-54-7
Molecular Weight:
515.25
Beilstein/REAXYS Number:
6543244
UNSPSC Code:
12171500
PubChem Substance ID:
329815467
NACRES:
NA.47

$162.00

In StockDetails

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Product Name

5-(Iodoacetamido)fluorescein, ≥90% (HPLC)

Quality Level

200

assay

≥90% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to orange

solubility

DMSO: 5 mg/mL

fluorescence

λex 492 nm; λem 515 nm (Mercaptoethanol adduct)
λex 493 nm; λem 522 nm (pH 9.2)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(NC(=O)CI)ccc45)c1

InChI

1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27)

InChI key

UATCLPJEZJKNHE-UHFFFAOYSA-N

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General description

5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative.

Application

5-(Iodoacetamido)fluorescein is used for the synthesis of fluorescently labeled organelles (nuclei and nuclear matrices), proteins and peptides that include enzymes.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW3411
MKCV1777
MKCT9233
MKCV3491
MKCV3490

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Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase.
Tyson PA
The Journal of Biological Chemistry, 264, 726-726 (1989)
Multisite phosphorylation of the Saccharomyces cerevisiae filamentous growth regulator Tec1 is required for its recognition by the E3 ubiquitin ligase adaptor Cdc4 and its subsequent destruction in vivo.
Bao MZ
Eukaryotic Cell, 9, 31-31 (2010)
Meng M Rowland et al.
Nucleic acids research, 42(14), 9295-9303 (2014-07-14)
The DNA backbone is often considered a track that allows long-range sliding of DNA repair enzymes in their search for rare damage sites in DNA. A proposed exemplar of DNA sliding is human 8-oxoguanine ((o)G) DNA glycosylase 1 (hOGG1), which
D Chhabra et al.
Biophysical journal, 89(3), 1902-1908 (2005-07-05)
Actin is the principal component of microfilaments. Its assembly/disassembly is essential for cell motility, cytokinesis, and a range of other functions. Recent evidence suggests that actin is present in the nucleus where it may be involved in the regulation of
Gábor Glatz et al.
The Journal of biological chemistry, 288(12), 8596-8609 (2013-02-06)
Mitogen-activated protein kinase (MAPK) activation depends on a linear binding motif found in all MAPK kinases (MKK). In addition, the PB1 (Phox and Bem1) domain of MKK5 is required for extracellular signal regulated kinase 5 (ERK5) activation. We present the
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