I8640
IgG from human serum
technical grade, ≥80% (SDS-PAGE), buffered aqueous solution
Synonym(s):
Human IgG
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10 MG
$43.10
50 MG
$135.00
100 MG
$232.00
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10 MG
$43.10
50 MG
$135.00
100 MG
$232.00
About This Item
application:
citations:
24
Recommended Products
conjugate
unconjugated
Quality Level
grade
technical grade
assay
≥80% (SDS-PAGE)
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis among many others[1]. IgG from human serum can be used as an economical alternative for reagent grade immunoglobulins where high purity is not essential. It can also be used in immunoelectrophoresis. The product is reactive in humans.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections[2]
Human IgG is purified from normal human serum by fractionation.
Human IgG is purified from normal human serum by fractionation.
Application
Purified human IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Technical grade human IgG may be used as an economical alternative to the reagent grade immunoglobulins, in applications where high purity is not required. IgG from human serum was used as ELISA standard in various studies.[3][4][5] A concentration range of 200 μg/ml to 1 mg/ml of human IgG was used for blocking in flow cytometry.[6][7][8]
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Silvia C W Ling et al.
Haematologica, 97(1), 64-72 (2011-10-14)
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Gerlândia Neres Pontes et al.
FEMS immunology and medical microbiology, 47(3), 405-413 (2006-07-29)
We evaluated the ability of human maternal and cord serum antibodies to protect mice challenged with live Escherichia coli serotype O6:K2ac (E. coli O6). Mice received paired maternal or cord serum pools before a challenge with E. coli O6 to
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J A Gebe et al.
The Journal of biological chemistry, 272(10), 6151-6158 (1997-03-07)
CD5 and CD6, two type I cell surface antigens predominantly expressed by T cells and a subset of B cells, have been shown to function as accessory molecules capable of modulating T cell activation. Here we report the cloning of
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