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GE80-6484-51

2-D Clean-Up Kit

Cytiva 80-6484-51

Synonym(s):

2D Clean-Up Kit

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About This Item

UNSPSC Code:
41105323
NACRES:
NA.25

feature

wetted part: no

manufacturer/tradename

Cytiva 80-6484-51

packaging

pkg of 50 samples

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General description

2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

Treatment of the sample with 2-D Clean-Up Kit can improve the quality of 2-D electrophoresis results, reducing streaking, background staining, and other artifacts due to interfering contaminants. The kit can make 2-D analysis possible for samples that are otherwise too dirty or too dilute.

The 2-D Clean-Up Kit procedure uses precipitant and co-precipitant in combination to quantitatively precipitate proteins. Precipitated proteins are pelleted by centrifugation and the precipitate is further washed to remove nonprotein contaminants. After a second centrifugation, the resultant pellet is resuspended into denaturing sample solution for first-dimension IEF.

The procedure can be completed in 90 min and does not result in spot gain or loss. The kit contains sufficient reagents to process 50 samples of up to 100 μ each. The procedure can be scaled up for larger volumes or more dilute samples.

2-D Clean-Up Kit increases labeling efficiency and protein abundance when using 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE).

Application

Automated identification and quantitation of proteins and peptides using LC-MS/MS.

Features and Benefits

  • Improves the quality of 2-D electrophoresis results by removing interfering contaminants
  • Quantitative precipitation separates proteins from detergents, salts, lipids, phenolics and nucleic acids
  • Delivers quantitative yield in about 90 min.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
The wash buffer should be placed at -20 °C at least 1 h before use and may be s-red in a -20 °C freezer

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Environment
GHS09

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Danger

Storage Class

8B - Non-combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Precipitation Procedures

This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.

Protocols

Desalting Samples with Mini Dialysis Kit

Mini Dialysis Kit from Cytiva minimizes sample handling and loss, reducing streaking on 2-D gels from contaminants.

Processing Small Tissue or Cell Samples using Sample Grinding Kit

Sample Grinding Kit from Cytiva is designed to disrupt cell or tissue samples for protein extraction.

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.

Western Blotting Sample Preparation

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service