C7275
Concanavalin A from Canavalia ensiformis (Jack bean)
Type V, lyophilized powder
Synonym(s):
Con A
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About This Item
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type
Type V
form
lyophilized powder
potency
≤64 μg per mL agglutination activity
impurities
Carbohydrate, essentially free
salt, essentially free
≤0.16 wt. % Ca
≤0.23 wt. % Mn
Storage temp.
−20°C
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Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Quality
Highly purified.
Preparation Note
Produced from whole Jack beans (J0250) using the method of Sophianopoulos.[1]
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
signalword
Danger
hcodes
Hazard Classifications
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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