4-Chloro-1-naphthol is a substrate for HRP in a reaction that results in a colored precipitate. It is commonly used for colorimetric detection and visualization of proteins in western blotting.
Application
Suitable for visualizing protein-peroxidase conjugates in western blotting.
Components
This product contains 0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.
Other Notes
0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.
Principle
Enzymes such as HRP convert 4-Chloro-1-naphthol to a purple-colored precipitate on the blot that is readily visible to the eye.
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