47860

Basic Fuchsin

for microscopy (Bact., Bot., Hist.), indicator (pH 1.0-3.1)

• Synonym(s): Basic Violet 14, Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2-methyl hydrochloride, Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2-methyl
• Empirical Formula (Hill Notation): C₂₀H₂₀ClN₃
• CAS Number: 632-99-5
• Molecular Weight: 337.85
• Colour Index Number: 42510
• UNSPSC Code: 12171500
• PubChem Substance ID: 24871527
• NACRES: NA.47

Properties

• grade: for microscopy (Bact., Bot., Hist.); indicator (pH 1.0-3.1)
• application(s): environmental
• SMILES string: NC1=CC=C(C(C2=CC(C)=C(N)C=C2)=C3C=CC(C=C3)=N)C=C1.[Cl]
• InChI: 1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,23H,21-22H2,1H3;1H
• InChI key: AXDJCCTWPBKUKL-UHFFFAOYSA-N

Description

General description

Basic fuchsin, a triphenylmethane dye is commonly a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II (no CI number) and new fuchsine (CI 42520). Basic fuchsin is widely used in tissue-specific staining of biological and coloring several materials including orlon, cotton, leather, paper etc. It is also used to stain collagen, muscle and mitochondria.

Application

Basic Fuchsin has been used in several biological tests including:

• Acid-fast staining with carbol-fuchsine
• Periodic Acid-Schiff method
• Feulgen technique for DNA staining
• ENDO culture medium for distinguishing between the bacteria Escherichia coli and Aerobacter aerogenes
• Leifson′s method for bacterial flagella staining.

Biochem/physiol Actions

Basic fuchsin possesses anesthetic, bactericidal (gram-positive), and fungicidal properties.

Analysis Note

homologous mixture of dyes, contains dominantly (~35-40%) Basic Fuchsin (C.I. 42510)

Safety Information

• pictograms: GHS08
• signalword: Warning
• hcodes: H351
• pcodes: P201 - P308 + P313
• Hazard Classifications: Carc. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges