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Sigma-Aldrich

N-(3-Maleimidopropionyl)biocytin

≥75% (HPLC)

Synonym(s):

MPB, Nε-Biotinyl-Nα-(3-maleimidopropionyl)-L-lysine

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About This Item

Empirical Formula (Hill Notation):
C23H33N5O7S
CAS Number:
102849-12-7
Molecular Weight:
523.60
MDL number:
MFCD16661254
UNSPSC Code:
12352200
PubChem Substance ID:
329759807
NACRES:
NA.25

Quality Level

100

assay

≥75% (HPLC)

SMILES string

OC(=O)[C@H](CCCCNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)NC(=O)CCN3C(=O)C=CC3=O

InChI

1S/C23H33N5O7S/c29-17(7-2-1-6-16-21-15(13-36-16)26-23(35)27-21)24-11-4-3-5-14(22(33)34)25-18(30)10-12-28-19(31)8-9-20(28)32/h8-9,14-16,21H,1-7,10-13H2,(H,24,29)(H,25,30)(H,33,34)(H2,26,27,35)/t14-,15-,16-,21-/m0/s1

InChI key

KWNGAZCDAJSVLC-OSAWLIQMSA-N

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Application

N-(3-Maleimidopropionyl)biocytin (MPB) is a versatile biotinylating reagent and thiol-specific probe that can detect protein SH groups on dot blots in the fmole range. MPB can be used for protein blotting, enzyme immunoassay, protein immobilization and various cytochemical procedures.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB9485
BCBR2131V
BCBQ9251V
BCBK2206V
BCBP1646V

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E Roffman et al.
Biochemical and biophysical research communications, 136(1), 80-85 (1986-04-14)
Amino groups, sulfhydryl groups or oxidation-induced aldehydes on erythrocyte membrane proteins and/or glycoproteins, were reacted with biotinyl N-hydroxysuccinimide ester (BNHS), 3-(N-maleimido-propionyl) biocytin (MPB) or biocytin hydrazide (BCHZ), respectively. The detergent-lysed biotinylated samples were subjected to SDS-polyacrylamide gel electrophoresis, and the
E A Bayer et al.
Analytical biochemistry, 161(2), 262-271 (1987-03-01)
Various strategies for the use of 3-(N-maleimido-propionyl) biocytin (MPB) as a general label for distinguishing between protein sulfhydryls and disulfides on blot transfers are presented. In the first approach, endogenous SH groups in proteins were labeled directly with MPB. For
T Wada et al.
The Journal of biological chemistry, 274(24), 17353-17357 (1999-06-08)
Cysteine mutagenesis and surface labeling has been used to define more precisely the transmembrane spans of subunit a of the Escherichia coli ATP synthase. Regions of subunit a that are exposed to the periplasmic space have been identified by a
Shin-Ichi Akanuma et al.
Drug metabolism and pharmacokinetics, 34(4), 239-246 (2019-06-09)
It has been known that organic anion-transporting polypeptides (Oatps) involve hepatic transports several organic anionic compounds and drugs. This study aimed to investigate sulforhodamine-101 (SR-101) distribution in the rat liver, determine the molecules responsible for the distribution, and delineate the
Feng Pan
Cells, 8(7) (2019-06-30)
Myopia is a substantial public health problem worldwide. Although it is known that defocused images alter eye growth and refraction, their effects on retinal ganglion cell (RGC) signaling that lead to either emmetropization or refractive errors have remained elusive. This
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