44585
Driselase™ from Basidiomycetes sp.
powder, light brown, ≥0.8 U/mg
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About This Item
Recommended Products
form
powder
specific activity
≥0.8 U/mg
color
light brown
storage temp.
2-8°C
Unit Definition
1 U corresponds to the amount of enzyme which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C; further enzyme activities: pectinase, xylanase, dextranase, laminarinase, amylase, protease, macerating and lytic activity; commonly used concentration: 1.0-2.5 %
Preparation Note
Preparation of protoplasts:
Place 0.2 g driselase in a conical flask, dissolve in 10 mL 4 M mannitol and adjust the pH with 0.1N HCl to 5.2. Suspend 1 g of cultured cells in the solution. When the enzyme is allowed to react with the cells for 1-2 hours while gently shaking the flask in a water bath at 30°C, most of the cells used as materials lose the cell walls and become spherical protoplasts.
Place 0.2 g driselase in a conical flask, dissolve in 10 mL 4 M mannitol and adjust the pH with 0.1N HCl to 5.2. Suspend 1 g of cultured cells in the solution. When the enzyme is allowed to react with the cells for 1-2 hours while gently shaking the flask in a water bath at 30°C, most of the cells used as materials lose the cell walls and become spherical protoplasts.
Other Notes
For the preparation of protoplasts; Analysis of pre-mRNA processing in transfected plant protoplasts
Legal Information
Driselase is a trademark of ASKA Animal Health Co. Ltd.
Replaced by
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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C.E. Flick et al.
Zeitschrift fur Pflanzenphysiologie, 109, 379-379 (1983)
Analysis of pre-mRNA processing in transfected plant protoplasts.
G J Goodall et al.
Methods in enzymology, 181, 148-161 (1990-01-01)
F. Hoffmann
Protoplasma, 107, 387-387 (1981)
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