ST1699

Anti-NDE1 Mouse mAb (2G11-1C11)

liquid, clone 2G11-1C11, Calbiochem^®

• Synonym(s): Anti-nudE, Anti-Nuclear Distribution Gene E Homolog1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2G11-1C11, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NDE1(54820)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDE1 protein.

Recognizes the ~40-45 kDa NDE1 protein in HeLa cells.

This Anti-NDE1 Mouse mAb (2G11-1C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of NDE1.

Immunogen

Full-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein

Application

ELISA (see coments)
Immunoblotting (1-4 g/ml)
Immunocytochemistry (10 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells

Other Notes

Bradshaw, N.J., et al. 2009. Neurosci. Lett.449, 228.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg