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Key Documents

MAB2630

Sigma-Aldrich

Anti-KRIT-1 Antibody, clone 15

clone 15B2, from mouse

Synonym(s):

Cerebral cavernous malformations 1 protein, Krev interaction trapped 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

15B2, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KRIT1(889)

General description

Cerebral cavernous malformation (CCM), a disease associated with defective endothelial junctions, results from autosomal dominant CCM1 mutations that cause a loss of KRIT-1 protein function. CCM1 has been identified as the KRIT-1 gene. The protein product, KRIT-1, also known as Krev interaction trapped protein 1, is a microtubule-associated protein involved in endothelial cell-cell and cell-extracellular matrix interactions. KRIT-1 binds to Rap1 that maintains the integrity of endothelial junctions. It is reported that KRIT-1 colocalizes and is physically associated with junctional proteins via its band 4.1/ezrin/radixin/moesin (FERM) domain, (Glading, 2007).

Specificity

The antibody recognizes KRIT-1.

Immunogen

Epitope: FERM domain
GST-tagged recombinant protein corresponding to human KRIT-1 at and around the FERM domain.

Application

Anti-KRIT-1 Antibody, clone 15 detects level of KRIT-1 & has been published & validated for use in IC, IP & WB.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Quality

Immunocytochemistry Analysis: 1:500 dilution of this antibody detected KRIT-1 in HeLa, A431, and HUVECs.

Target description

~ 84 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa, A431, and HUVECs.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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