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Sigma-Aldrich

Atto 550 Protein Labeling Kit

BioReagent, suitable for fluorescence

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

product line

BioReagent

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 544 nm; λem 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

suitability

suitable for fluorescence

storage temp.

2-8°C

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General description

This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550 Protein Labeling Kit contains Atto 550 succinimidyl ester which enables conjugation of Atto 550 to the primary amino groups of the proteins and other molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

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Andrew H J Salmon et al.
Journal of the American Society of Nephrology : JASN, 23(8), 1339-1350 (2012-07-17)
Patients with albuminuria and CKD frequently have vascular dysfunction but the underlying mechanisms remain unclear. Because the endothelial surface layer, a meshwork of surface-bound and loosely adherent glycosaminoglycans and proteoglycans, modulates vascular function, its loss could contribute to both renal
Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Yixiong Wang et al.
Nature communications, 13(1), 3794-3794 (2022-07-02)
The DEAD box protein DDX1, previously associated with 3'-end RNA processing and DNA repair, forms large aggregates in the cytoplasm of early mouse embryos. Ddx1 knockout causes stalling of embryos at the 2-4 cell stages. Here, we identify a DDX1-containing
Antibody transfection into neurons as a tool to study disease pathogenesis.
Douglas JN, Gardner LA, et al.
Journal of Visualized Experiments, 26, 4154-4154 (2012)
Michel Frigoli et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 15(33), 8319-8330 (2009-07-04)
Core-shell type dual fluorescent nanoparticles (NPs) in the 16 nm diameter range with a selective ligand (cyclam) attached to the surface and two fluorophores--9,10-diphenyl-anthracene (donor, D) and pyrromethene PM 567 (acceptor, A)--embedded within the polymer core were synthesized and their
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Atto & Tracy Dyes for Protein Labeling

Protein labeling kits with Atto and Tracy dyes provide easy fluorescent labeling of purified proteins, enzymes, and antibodies.

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