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MAB1585

Sigma-Aldrich

Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2

culture supernatant, clone 5A3.2, Chemicon®

Synonym(s):

Anti-ATITHS, Anti-BRN3A, Anti-Oct-T1, Anti-RDC-1, Anti-brn-3A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

5A3.2, monoclonal

species reactivity

chicken, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_006237.3

UniProt accession no.

Q01851

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... POU4F1(5457)

Specificity

Recognizes Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice.

Immunogen

Amino acids 186-224 of Brn-3a fused to the T7 gene 10 protein (Xiang et al., 1995).
Epitope: POU-domain protein

Application

Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2 detects level of Brn-3a & has been published & validated for use in IH & WB.
Immunohistochemistry: 1:5-1:125

Western blot: 1:50-1:100

Optimal working dilutions must be determined by end user.

Target description

46 kDa

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Combinatorial expression of Brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis.
Badea, TC; Williams, J; Smallwood, P; Shi, M; Motajo, O; Nathans, J
The Journal of Neuroscience null
Sustained ocular hypertension induces dendritic degeneration of mouse retinal ganglion cells that depends on cell type and location.
Feng, L; Zhao, Y; Yoshida, M; Chen, H; Yang, JF; Kim, TS; Cang, J; Troy, JB; Liu, X
Investigative Ophthalmology & Visual Science null
The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
Zelinka, CP; Scott, MA; Volkov, L; Fischer, AJ
Testing null
Embryonic retinal cells and support to mature retinal neurons.
Stanke, JJ; Fischer, AJ
Investigative Ophthalmology & Visual Science null
Masahiro Miyake et al.
Nature communications, 6, 6689-6689 (2015-04-01)
Myopia can cause severe visual impairment. Here, we report a two-stage genome-wide association study for three myopia-related traits in 9,804 Japanese individuals, which was extended with trans-ethnic replication in 2,674 Chinese and 2,690 Caucasian individuals. We identify WNT7B as a
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