AB15282
Anti-Cone Arrestin Antibody
Chemicon®, from rabbit
Synonym(s):
Anti-Arrestin-C
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
mol wt
antibody estimated mol wt ~46 kDa
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Arr3(170735)
rat ... Arr3(171107)
General description
Arrestin-C (UniProt: Q9EQP6; also known as Cone arrestin, cArr, Retinal cone arrestin-3) is encoded by the Arr3 gene (Gene ID: 170735) in murine species. Arrestins are a superfamily of multi-functional proteins that that regulate signaling and trafficking of the majority of G-protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins. Arrestin-C, a disulfide-linked, homodimeric protein, is predominantly found in Inner and outer segments, and the inner plexiform regions of the retina. It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision. Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes. Several structural elements are reported to contribute to arrestin binding. The C-terminal acidic region serves a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor. The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region. Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity. Mutations in ARR3 gene in humans have been linked to X-lined myopia 26 that is characterized by typical tigroid fundus changes commonly seen in early onset high myopia. (Ref.: Gurevich, VV., et al. (2018). Protein Cell. 9; 986-1003; Xiao, X., et al. (2016). Mol. Vis. 22; 1257-1266).
Specificity
This rabbit polyclonal antibody detects Cone Arrestin. It targets an epitope within 12 amino acids from the C-terminal region.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of mouse Cone Arrestin.
Application
Tested ApplicationsImmunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Arrestin-C in Rat retina, Mouse retina and Mouse brain tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-Cone Arrestin, Cat. No. AB15282, is a rabbit polyclonal antibody that detects Arrestin-C and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
Quality
Evaluated by Western Blotting in Mouse retina tissue lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected Cone Arrestin in Mouse retina tissue lysate.
Target description
~41.9 kDa
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sensitivity and kinetics of signal transmission at the first visual synapse differentially impact visually-guided behavior.
Sarria, I; Pahlberg, J; Cao, Y; Kolesnikov, AV; Kefalov, VJ; Sampath, AP; Martemyanov, KA
eLife null
Phospholipid flippase ATP8A2 is required for normal visual and auditory function and photoreceptor and spiral ganglion cell survival.
Coleman, JA; Zhu, X; Djajadi, HR; Molday, LL; Smith, RS; Libby, RT; John, SW; Molday, RS
Journal of Cell Science null
Mei Chen et al.
PloS one, 8(4), e61381-e61381 (2013-05-03)
Previous studies have shown that CCL2/CX3CR1 deficient mice on C57BL/6N background (with rd8 mutation) have an early onset (6 weeks) of spontaneous retinal degeneration. In this study, we generated CCL2(-/-)CX3CR1(GFP/GFP) mice on the C57BL/6J background. Retinal degeneration was not detected
Katharina Kranz et al.
PloS one, 8(2), e57163-e57163 (2013-03-08)
Retinitis pigmentosa (RP) relates to a group of hereditary neurodegenerative diseases of the retina. On the cellular level, RP results in the primary death of rod photoreceptors, caused by rod-specific mutations, followed by a secondary degeneration of genetically normal cones.
Susanne F Koch et al.
The Journal of clinical investigation, 125(9), 3704-3713 (2015-08-25)
Hereditary retinal degenerative diseases, such as retinitis pigmentosa (RP), are characterized by the progressive loss of rod photoreceptors followed by loss of cones. While retinal gene therapy clinical trials demonstrated temporary improvement in visual function, this approach has yet to
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