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Key Documents

64571-M

Sigma-Aldrich

Wright-Giemsa Fuccillo Stain Modification

Wright-Giemsa Fuccillo Stain Modification

Harleco®, suitable for hematology

Synonym(s):

Hematology Stain, Hematology Solutions

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About This Item

UNSPSC Code:
41116121

form

liquid

Quality Level

300

manufacturer/tradename

Harleco®

mp

-98 °C

density

0.791 g/cm3

λmax

520-530 nm (Eosin)
640-650 nm (Azure)

application(s)

diagnostic assay manufacturing
hematology
hematology ((contains Methanol))

storage temp.

15-25°C

General description

HARLECO® Polychromatic stain for peripheral blood and bone marrow smears

Application

HARLECO® Polychromatic stain for peripheral blood and bone marrow smears
Prepared from dyes certified by the Biological Stain Commission (BSC)
For In-Vitro Diagnostic Use.
For Professional Use.
Polychromatic stain for peripheral blood and bone marrow smears. Used for red blood cell morphology and white blood cell differentiation.

Legal Information

HARLECO is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

Certificates of Analysis (COA)

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Questions

  1. What is the mixture of stain to buffer for the Wright Giemsa Fucillo solution?

    1 answer

    1. As per the instructions located on page 1 of the Instructions for Use, the stain buffer mix is prepared by combining 50 ml of the Wright Giemsa Fucillo stain with 250 ml of buffer. Depending on user preference, the pH of the buffer should be either pH 6.4 or 6.8. It is conceivable that some users might prefer a slightly more alkaline pH buffer. The stain buffer mixture should remain effective for at least 3 hours, but it is possible that it might remain effective for as long as 24 hours or perhaps slightly longer. When the stain quality begins to deteriorate, the stain buffer mix should be replaced.

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Reviews

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  1. Montreal, Qc
    • Reviews 2
    • Vote 1
    5 out of 5 stars.

    Beautiful stain

    We have great results with this stain for a manual technique. It works very well with the phosphate buffer with a pH of 6,4.

    Helpful?

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