Product 109204 does not have specific claims for staining malarial parasites. The Instructions For Use mention staining Helicobacter pylori, but not malaria, as the product is approved for In Vitro Diagnostic Use. The Giemsa solution 109204 is an unbuffered solution. The instructions advise using buffers at pH 6.4, 6.8, or 7.2, depending on customer preference. The resulting colors obtained with the Giemsa solution largely depend on its pH.
1.09204
Giemsa′s azur eosin methylene blue solution
Giemsa′s azur eosin methylene blue solution
for microscopy
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100 ML
$48.40
500 ML
$120.00
1 L
$206.00
2.5 L
$357.00
25 L
$3,410.00
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100 ML
$48.40
500 ML
$120.00
1 L
$206.00
2.5 L
$357.00
25 L
$3,410.00
About This Item
UNSPSC Code:
41116130
NACRES:
NA.05
Recommended Products
grade
for microscopy
Quality Level
form
liquid
autoignition temp.
455 °C
composition
C.I. 45380, 2.4 g/L
C.I. 52015 + Azure, 4.1 g/L
contains CH3OH
expl. lim.
5.5-44 % (v/v) Methanol()
IVD
for in vitro diagnostic use
technique(s)
microbe id | staining: suitable
pH
6.1-7.0 (20 °C in H2O, undiluted)
bp
>65 °C/1013 hPa
transition temp
flash point 18 °C
density
0.99 g/cm3 at 20 °C
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
General description
Giemsa′s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa′s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald′s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa′s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Application
For microscopy. For the staining of blood and bone-marrow smears, paraffin sections and clinical-cytological specimens
Analysis Note
For staining results, please refer to the Instructions for Use link under the Documents Section below.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1
target_organs
Eyes,Central nervous system
Storage Class
3 - Flammable liquids
wgk_germany
WGK 2
flash_point_f
64.4 °F
flash_point_c
18 °C
Certificates of Analysis (COA)
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Can product 1092040500 be used as a malarial stain? The specified pH range for this product is 6.0 - 8.2 (H₂O, 20 °C) (undiluted). How can the pH be adjusted to 7.2?
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