251520-M

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• Synonym(s): DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
• Empirical Formula (Hill Notation): C₂₅H₁₈F₂N₂O₇
• CAS Number: 254109-22-3
• Molecular Weight: 496.42
• MDL number: MFCD16872050
• UNSPSC Code: 12352204
• NACRES: NA.25

Properties

• Quality Level: 100
• assay: ≥98% (HPLC)
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: pale yellow
• fluorescence: λ_ex ~500 nm; λ_em ~515 nm
• storage temp.: −20°C
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• InChI key: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Description

General description

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

Biochem/physiol Actions

Primary Target
Nitric oxide (NO) fluorescent indicator

Warning

Toxicity: Irritant (B)

Physical form

A 5 mM solution in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

Other Notes

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

Legal Information

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: 188.6 °F - closed cup - (Dimethylsulfoxide)
• flash_point_c: 87 °C - closed cup - (Dimethylsulfoxide)