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Key Documents

MAK454

Sigma-Aldrich

Nitric Oxide Assay Kit

Sufficient for 100 colorimetric tests

Synonym(s):

NO Detection Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

application(s)

cosmetics
food and beverages
pharmaceutical

detection method

colorimetric

relevant disease(s)

cardiovascular diseases; infectious diseases; neurological disorders

storage temp.

−20°C

General description

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules.

Application

The Nitric Oxide Assay Kit may be used for:
  • Cardiovascular Research
  • Neurological Research
  • Oxidative Stress Research

Features and Benefits

High Sensitivity and Accuracy: Detect Nitric Oxide (NO) levels ranging from 0.6 - 200 μM with a short 30 minute procedure, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

The kit is suitable for the quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism in plasma, serum, urine, tissue, cells and foods.

Principle

Simple, direct and automation-ready procedures for measuring NO are becoming popular in research and drug discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3- as a measure for NO level. The Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 30 minutes.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

CorrosionEnvironment

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Storage Class

8A - Combustible corrosive hazardous materials


Certificates of Analysis (COA)

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