03-0180-00

SMC^® Human VEGF-A High Sensitivity Immunoassay Kit

• Synonym(s): VEGF-A Single Molecule Counting Kit, VEGF-A ultra-sensitive immunoassay, Vascular endothelial growth factor A immunoassay kit, Vascular permeability factor immunoassay kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• species reactivity: canine, nonhuman primates, feline
• manufacturer/tradename: SMC^®
• assay range: sensitivity: 0.2 pg/mL; (Lower Limit of Quantification); inter-assay cv: <20%; intra-assay cv: <15%; (Intra-assay n=20; Inter-assay n=5)
• technique(s): single molecule counting: suitable
• storage temp.: 2-8°C

Description

Specificity

Species Reactivity:
Although VEGF was detectable in non-human primate, feline, and canine plasma, the assay has not been validated for these species.

Cross Reactivity:
The SMC^® Human VEGF-A High Sensitivity Immunoasay Kit was not found to be cross-reactive with the following:

• rhVEGF-B
• rhVEGF-C
• rhVEGF-D

Accuracy:

• Minimum of 5 serum samples spiked with average recovery of 103% with a range of 95-110%
• Minimum of 5 plasma samples spiked with average recovery of 93% with a range of 80-107%

Application

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC^® Human VEGF-A High Sensitivity Immunoassay Kit unparalleled performance in the quantification of VEGF-A in human serum and plasma.

This new streamline kit has the following features including:

• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

Research Category:
Inflammation & Immunology

Research Sub-Category:

• Inflammatory Disease
• Immune Response
• Autoimmune disease

Packaging

96 well assay

Components

• Assay Buffer
• VEGF Coated Beads
• Standard Diluent
• VEGF Detection Antibody
• VEGF Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H412
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects