L-047

Lactoferrin (from bovine milk)

certified reference material, pkg of 50 mg, Cerilliant^®

• CAS Number: 146897-68-9
• UNSPSC Code: 41116107
• NACRES: NA.24

Properties

• grade: certified reference material
• Quality Level: 300
• form: solid
• mol wt: 82401 Da
• feature: Snap-N-Spike^®/Snap-N-Shoot^®
• packaging: pkg of 50 mg
• manufacturer/tradename: Cerilliant^®
• technique(s): liquid chromatography (LC): suitable
• application(s): food and beverages
• format: neat
• storage temp.: −20°C
• InChI: 1S/C141H224N46O29S3/c1-11-77(6)111-133(212)186-112(80(9)189)134(213)183-106(130(209)184-110(76(4)5)132(211)176-97(49-32-61-161-141(155)156)120(199)170-93(45-28-57-157-137(147)148)116(195)166-78(7)113(192)180-103(136(215)216)66-82-36-16-13-17-37-82)74-219-218-73-105(182-123(202)90(42-22-25-54-142)167-114(193)87(145)65-81-34-14-12-15-35-81)129(208)173-95(47-30-59-159-139(151)152)118(197)171-96(48-31-60-160-140(153)154)122(201)178-102(68-84-70-163-89-41-21-19-39-86(84)89)127(206)174-98(51-52-108(146)190)124(203)179-101(67-83-69-162-88-40-20-18-38-85(83)88)126(205)172-94(46-29-58-158-138(149)150)119(198)175-99(53-63-217-10)125(204)169-91(43-23-26-55-143)117(196)168-92(44-24-27-56-144)121(200)177-100(64-75(2)3)115(194)164-71-109(191)165-79(8)135(214)187-62-33-50-107(187)131(210)181-104(72-188)128(207)185-111/h12-21,34-41,69-70,75-80,87,90-107,110-112,162-163,188-189H,11,22-33,42-68,71-74,142-145H2,1-10H3,(H2,146,190)(H,164,194)(H,165,191)(H,166,195)(H,167,193)(H,168,196)(H,169,204)(H,170,199)(H,171,197)(H,172,205)(H,173,208)(H,174,206)(H,175,198)(H,176,211)(H,177,200)(H,178,201)(H,179,203)(H,180,192)(H,181,210)(H,182,202)(H,183,213)(H,184,209)(H,185,207)(H,186,212)(H,215,216)(H4,147,148,157)(H4,149,150,158)(H4,151,152,159)(H4,153,154,160)(H4,155,156,161)/t77-,78-,79-,80+,87-,90-,91-,92-,93-,94-,95-,96-,97-,98-,99-,100-,101-,102-,103-,104-,105-,106-,107-,110-,111-,112-/m0/s1
• InChI key: PHEGMCHJJYMADA-IKGCZBKSSA-N

Description

General description

Lactoferrin is a multifunctional glycoprotein that belongs to the transferrin family of proteins. It is mostly found in the mucosal secretions of mammals, such as tears, saliva, and bile but is abundantly present in milk.

Lactoferrin, a wonder molecule, holds significant importance in promoting health and managing pathology throughout all stages of life. This iron binding milk protein also helps to balance iron levels in the body. Lactoferrin (from bovine milk) is a certified reference material. The certified property value is linked to National Institute of Standards and Technology (NIST) through calibration verification achieved using weights traceable to NIST standards.

Application

This full-length protein CRM is suitable as starting material for use in calibrators or controls for a variety of LC-MS/MS testing applications including allergen testing, infant formula testing, dietary or nutritional and diagnostic testing application.The CRM can also be used as follows:

• Development of a three immunoassay— indirect competitive ELISA, colloidal gold strip, and AuNFs-based strip, to determine lactoferrin in real cow milk samples using an IgG secreted by hybridoma LF8
• Determination of bovine lactoferrin in milk and milk product samples by a validated competitive enzyme-linked immunosorbent assay (ELISA) based method
• Quantification of lactoferrin in dairy products by immunoaffinity magnetic purification combined with high-performance liquid chromatography-fluorescence (HPLC-FL) detection
• Quantitative analysis of bovine lactoferrin in sterilized milk, modified milk, fermented milk, infant formula, adult formula, rice cereal, vitamin function drink, and protein powder samples by HPLC coupled with diode array detection (DAD)
• Development and validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) based assay for the measurement of bovine lactoferrin in infant formulae samples

Features and Benefits

• Balances and National Institute of Standards and Technology (NIST) traceable weights are calibrated by ISO 17025 accredited vendors.
• The material has undergone comprehensive identification and characterization using various analytical methods.
• Long-term stability has been evaluated under freezer storage conditions (-10 °C to -25 °C).

Preparation Note

• Weigh the material before use.
• Determine the amount to be weighed using the certified property value.
• The material is hygroscopic, so minimize moisture exposure during weighing.
• A minimum sample size of 20 mg is recommended for quantitative applications.
• If a smaller sample size is used, increase the uncertainty by a factor of 2.

Other Notes

This product′s purpose is for testing. THIS PRODUCT IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE. Not recommended for human or animal consumption.

Legal Information

CERILLIANT is a registered trademark of Merck KGaA, Darmstadt, Germany

Snap-N-Shoot is a registered trademark of Cerilliant Corporation

Snap-N-Spike is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable