After reconstitution, it is advisable to aliquot and freeze the product T8158, Human Transferrin at -20°C for long-term storage or add NaN₃ and refrigerate it at 4°C for short-term storage. Stock solutions can remain stable for up to 1 year at -20°C or for up to 2 weeks at 4°C.
T8158
Transferrin human
powder, BioReagent, suitable for cell culture
Synonym(s):
Siderophilin, partially saturated
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100 MG
$151.00
1 G
$894.00
About This Item
Recommended Products
product line
BioReagent
Quality Level
assay
≥98%
form
powder
concentration
~25 mM
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
hepatitis and HIV antibody, none detected
solubility
H2O: 50 mg/mL
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
Gene Information
human ... TF(7018)
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General description
Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.[1]
Application
Transferrin human has been used:
- to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity[2]
- as a supplement in serum-containing/devoid medium for human thyroid cell culture[3]
- as a target glycoprotein to develop an in-gel hydrazinolysis method[4]
Biochem/physiol Actions
Transferrin mediates iron transportation from plasma to cells.[1] The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin.[1] The transferrin binds to specific cell surface receptors and are internalized by endocytosis.[5] Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded.[5][6] Transferrin is known to deliver iron to cells in a physiologically safe and effective form.
Other Notes
Partially iron-saturated
Preparation Note
Heat treated at 60 °C for ten hours.
Analysis Note
Purity by agarose electrophoresis.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Related product
Product No.
Description
Pricing
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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At pH 5.4 apotransferrin (iron-free transferrin) binds to cell-surface transferrin receptors to the same extent and with the same affinity as does diferric transferrin at pH 7.0. Apotransferrin is quickly dissociated from its receptor when the pH is raised to
Articles
Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.
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What is the correct method for storing item T8158, Human Transferrin, after reconstitution?
1 answer-
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We were wondering how to store the dissolved transferrin. We have frozen it at -80. If we thaw it can we can freeze it again,
1 answer-
Transferrin is fairly stable in solution. Non-sterile aqueous solutions may be stored at 4 °C for 5-10 days. Solutions may be filter-sterilized to extend solution stability. Various media supplements containing transferrin, such as ITS (Insulin/Transferrin/Selenium), are stable for up to 30 days at 4 °C and may be stored in frozen aliquots at -20 °C. for up to one year. Avoid repeated freeze/thaw cycles as this may contribute to precipitation occurring.
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How is this protein produced and what is the rest 2% impurity? Thanks!
1 answer-
The preparation method is considered proprietary. The residual impurities have not been identified. This product is tested for endotoxin and iron. Please see the Sample Certificates of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T8158-BULKBCCK4250.pdfHelpful?
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What is the carbohydrate content of human transferrin?
1 answer-
We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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