T5391
apo-Transferrin human
γ-irradiated, powder, BioXtra, suitable for cell culture, ≥98%
Synonym(s):
Human transferrin, Siderophilin
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About This Item
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Quality Level
sterility
γ-irradiated
product line
BioXtra
assay
≥98%
form
powder
mol wt
76-81 kDa
concentration
~25 mM
technique(s)
cell culture | mammalian: suitable
impurities
HIV and HBsAg, source material tested negative
iron, essentially free
solubility
H2O: 50 mg/mL
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TF(7018)
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General description
apo-Transferrin (aTF) human can be supplemented with iron or used to bind free iron present in media. It can be used to transport iron into mammalian cells.
Application
apo-Transferrin human has been used as a supplement:
- in Dulbecco′s modified Eagle medium/HamF12 (DMEM/F-12) to culture human adrenocortical carcinoma cells
- in DMEM/F-12 to culture neural precursor cells from mice
- in collecting medium NCSU23 to culture preantral follicles from porcine ovaries
Biochem/physiol Actions
apo-Transferrin (aTF) is an endogenous iron chelator and shows protective effects in the host by scavenging free iron and preventing the formation of toxic reactive oxygen species (ROS). It shows a therapeutic effect against thalassemia. After the release of iron, receptor-bound apo-transferrin reaches the cell surface instead of being transported to lysosomes for degradation. The cell surface has a neutral pH that promotes the release of apo-transferrin from its receptor, where it can again circulate and bind additional iron to undergo further rounds of iron delivery to cells.
Other Notes
Non-heme iron-transport protein.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificates of Analysis (COA)
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Articles
Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.
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